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  • 1.  Invitrogen Qubit 4 RNA Integrity & Quality

    Posted 03-29-2025 09:46
    snHello,

    My name is Bill Peyron Jr and I currently work as a Molecular Biologist at American Oncology Network in Fort Myers, Florida.  
    I am looking for any assistance on using the Invitrogen Qubit 4 Fluorometer for checking our RNA Integrity and Quality. Our Nanodrop Lite Spectrophotometer readings are in the range of 30-90 ng/ul and the 260/280 absorbance reading is above 2.00 showing a good quality of extracted RNA. When we tested 6 RNA specimens using the Qubit, we left the IQ reagents (stored at -20) and the RNA IQ standards 1,2 &3 (stored at -80) at room temperature for 30 minutes before testing.  We did get RNA IQ readings on three out of our six specimens: 59.6 ng/ul, 260/280= 2.01; 71.5 ng/ul, 260/280=2.02 and 62.4 ng/ul, 260/280= 2.00.  Three other specimens: 49.6 ng/ul, 260/280=2.00; 56.9 ng/ul, 260/280=2.01 and 52.4 ng/ul, 260/280=2.02 resulted in a measurement error.

    Any assistance in using the Qubit would be greatly appreciated.

    Bill Peyron, Jr., MB(ASCP)


  • 2.  RE: Invitrogen Qubit 4 RNA Integrity & Quality

    Posted 03-30-2025 00:47

    Hi William ,

    I'm Gerges , sequencing scientist. I'm happy to help guide you. Email me at Gerges.samirsmb@gmail.com.

    lets schedule a meeting and go through it together I believe I can help. 

    Regards,

    Gerges Nazir.



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    Gerges Samir Nazir
    Louisville KY
    (505)029-326
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    Original Message