Laboratory Professionals Member Community

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  • 1.  Expression Microdissection

    Posted 04-09-2024 13:40

    Hi!

    I am a senior tech (HTL) at a molecular company. I was wondering if anyone uses expression microdissection on FFPE samples for NGS testing. If you do, what are the challenges that you find and do you think they outweigh the benefit of specificity?

    Thank you for any help!



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    Kathleen Mitchell Supervisor/Lead Med Lab Scientist/MedTechnologist
    Oak Lawn IL
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  • 2.  RE: Expression Microdissection

    Posted 04-10-2024 15:54
    Hi Everyone,

    Our HRC Rehabilitation - Laboratory has three criteria that reflex Urine Culture. We do all Urinalysis complete w/ Microscopy reflex to Urine Culture.

    Below are reflex urine culture criteria:
    1. Leukocytes: positive 
    2. Nitrite : positive 
    3. WBCs: >5/ hog

    Only bacteria positive it could  be superficial contamination that will show normal flora in urine culture report. 

    Any one of the above criteria present in urinalysis complete that will also shows few- many bacteria in urinalysis microscopy. 

    Thank you!
    Nila Patel
    Laboratory and Radiology services Director